JavaScript Menu Courtesy of Milonic.com
[nucleotide sequence]     [protein sequence]     [ncbi BLAST]     [add construct]     [add ligand annotation]

TeR258   (not provided)

database refs UniProt: Q8DJY4 (Q8DJY4_THEEB)   |   NCBI gene: 1012276   |   refSeq: NP_681877.1
status X-Ray structure
structures 4IOH (Xray)   4IOH (Xray)  
properties length: 126 aa   |   mass: 13.64 kD   |   pI: 5.85   |   ext: 15470   |   organism: Thermosynechococcus elongatus
classes BIG: CRSH, PSI-Biology
projects CRSH set 1     CRSH    
best PDB match calculating ...
informatics Zn binding sites: no sites identified. CH type (data) CHDE type (data)
domain signaturesPF09996 details   BIG_415 details  
subsequence details   ie 100-200    
BLAST searches

Construct coverage schematic

Domain and UniProt Features
    BIG BIG_415
    PFAM PF09996
    {ECO:0000213|PDB:4IOH} domain_number-1
    {ECO:0000213|PDB:4IOH} domain_number-2
    {ECO:0000213|PDB:4IOH} domain_number-3

TeR258

       PDB seq homology



Constructs and Most Advanced Status
   
TeR258-21.*

   
TeR258-6-126-21.*



most recent record
4 years, 18 days ago --     X-Ray structure: 4ioh

2011-07-19    target: TeR258
2011-08-16    construct: TeR258-21.4
2015-01-07     ter258.023 @ Rutgers; construct sample
2011-09-19    expression [ 4, 4 ] : TeR258-21.4-ss
2011-09-27    expression [ 5, 5 ] : TeR258-21.4-SeMa
2011-10-03    purification [30.4 mg]: TeR258-21.4-SeMa-GF
2011-10-11     aggregation screening [dimer,35.35 kD,91.5%|heptamer,109.8 kD,4.5%]
2011-10-07     TeR258.011 @ HWI; 0.45 ml at 7.51 mg/mL
2011-10-07     TeR258.010 @ HWI; 0.45 ml at 14.24 mg/mL
2011-10-07     TeR258.009 @ Rutgers; 1 ml at 7.51 mg/mL
2011-10-07     TeR258.008 @ HWI; 0.45 ml at 6.81 mg/mL
2011-10-07     TeR258.007 @ HWI; 0.45 ml at 12.71 mg/mL
2011-10-07     TeR258.006 @ Rutgers; 1.5 ml at 12.71 mg/mL
2011-11-16     TeR258.016 @ Columbia; 1 ml at 8.15 mg/mL
2013-01-07     X-Ray structure: 4ioh (PDB)
2011-11-16     TeR258.017 @ HWI; 0.45 ml at 8.15 mg/mL
2015-01-07     TeR258.022 @ Rutgers; 0.5 ml at 12.71 mg/mL
2011-09-27    expression [ 4, 5 ] : TeR258-21.4-NC5b
2011-10-12    purification [82.9 mg]: TeR258-21.4-NC5b-Ni
2011-10-13    purification [55.5 mg]: TeR258-21.4-NC5b-GF
2011-11-14     TeR258.014 @ Rutgers; 0.04 ml at 0.932 mM
2011-11-21    HSQC [good]: TeR258.014.600MHz.20C
2011-11-14     TeR258.015 @ Rutgers; 1.75 ml at 0.932 mM
2011-09-27    expression [ 5, 5 ] : TeR258-21.4-SeMb
2011-10-03    purification [30.4 mg]: TeR258-21.4-SeMb-GF
2011-09-27    expression [ 3, 5 ] : TeR258-21.4-NC5a
2011-10-12    purification [79.7 mg]: TeR258-21.4-NC5a-Ni
2011-10-13    purification [53.3 mg]: TeR258-21.4-NC5a-GF
2011-08-16    construct: TeR258-6-126-21.2
2011-09-19    expression [ 5, 4 ] : TeR258-6-126-21.2-ss
2011-09-21    expression [ 5, 4 ] : TeR258-6-126-21.2-SeMa
2011-09-27    purification [19.6 mg]: TeR258-6-126-21.2-SeMa-GF
2011-10-04     aggregation screening [Aggregated,481.6 kD,50.6%]
2011-10-04     TeR258.002 @ Rutgers; 0.45 ml at 8 mg/mL
2011-10-04     TeR258.001 @ Rutgers; 1.55 ml at 8 mg/mL
2011-10-04     TeR258.003 @ Rutgers; 2 ml at 5.76 mg/mL
2011-10-04     TeR258.004 @ Rutgers; 0.45 ml at 5.76 mg/mL
2011-10-04     TeR258.005 @ Rutgers; 3 ml at 5.76 mg/mL
2011-09-21    expression [ 4, 5 ] : TeR258-6-126-21.2-NC5a
2011-10-03    purification [109.7 mg]: TeR258-6-126-21.2-NC5a-Ni
2011-10-03    purification [55.6 mg]: TeR258-6-126-21.2-NC5a-GF
2011-10-31     aggregation screening [monomer,15.27 kD,95.2%]
2011-12-02     ter258.020 @ Rutgers; 0.5 ml at 0.521 mM
2011-12-06    HSQC [good]: TeR258.020.600MHz.20C
2011-10-11     TeR258.012 @ Rutgers; 0.04 ml at 0.521 mM
2011-10-26    HSQC [good]: TeR258.012.600MHz.20C
2011-10-11     TeR258.013 @ Rutgers; 2.5 ml at 0.521 mM
2011-12-06     TeR258.021 @ UGA; 1 ml at 0.521 mM
2011-11-02    expression [ 5, 5 ] : TeR258-6-126-21.2-NCa
2011-11-28    purification [38.8 mg]: TeR258-6-126-21.2-NCa-Ni
2011-11-28    purification [39.7 mg]: TeR258-6-126-21.2-NCa-GF
2011-12-02     ter258.018 @ Rutgers; 0.3 ml at 0.49 mM
2011-12-06    HSQC [promising]: TeR258.018.600MHz.20C
2011-12-08    HSQC [promising]: TeR258.018.600MHz.25C
2011-12-02     TeR258.019 @ Rutgers; 1 ml at 0.49 mM
2011-11-02    expression [ 5, 5 ] : TeR258-6-126-21.2-NC5b
2011-11-28    purification [35.5 mg]: TeR258-6-126-21.2-NC5b-Ni
2011-11-28    purification [0.0 mg]: TeR258-6-126-21.2-NC5b-GF
2011-08-16    construct: TeR258-21.3
2011-09-19    expression [ 4, 4 ] : TeR258-21.3-ss
2011-08-16    construct: TeR258-6-126-21.1
2011-09-19    expression [ 5, 5 ] : TeR258-6-126-21.1-ss


Available samples:

SeMet samples
TeR258.011 @ HWI TeR258.006 @ TeR258.007 @ HWI TeR258.017 @ HWI TeR258.009 @ Rutgers
TeR258.022 @ TeR258.004 @ Rutgers TeR258.002 @ Rutgers TeR258.001 @ Rutgers TeR258.005 @ Rutgers
TeR258.016 @ Columbia TeR258.010 @ HWI TeR258.008 @ HWI TeR258.003 @ Rutgers


NC samples
TeR258.019 @ Rutgers
ter258.018 @ Rutgers


NC5 samples
TeR258.015 @ Rutgers TeR258.013 @ Rutgers ter258.020 @ Rutgers TeR258.014 @ Rutgers
TeR258.012 @ Rutgers TeR258.021 @ UGA