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WsR35   (not provided)

database refs UniProt: Q7MSS8 (Q7MSS8_WOLSU)   |   NCBI gene: 2555421   |   refSeq: NP_906430.1
status X-Ray structure
structures 4INA (Xray)  
properties length: 397 aa   |   mass: 44.83 kD   |   pI: 5.17   |   ext: 61310   |   organism: Wolinella succinogenes
classes BIG: CRSH, PSI-Biology
projects CRSH set 1     CRSH    
best PDB match calculating ...
informatics Zn binding sites: no sites identified. CH type (data) CHDE type (data)
domain signatures3.40.50.720 details   PF03435 details  
subsequence details   ie 100-200    
BLAST searches

Construct coverage schematic

Domain and UniProt Features
    CATH 3.40.50.720
    Sacchrp_dh_NADP domain_number-1
    PFAM PF03435
    Sacchrp_dh_C. {ECO:0000259|Pfam:PF16653} domain_number-1

WsR35

       PDB seq homology



Constructs and Most Advanced Status
   
WsR35-15.*

   
WsR35-21.*

   
WsR35-TEV.*



most recent record
4 years, 21 days ago --     X-Ray structure: 4ina

2011-07-18    target: WsR35
2011-08-16    construct: WsR35-21.2
2015-02-16     wsr35.011 @ Rutgers; construct sample
2011-09-19    expression [ 5, 5 ] : WsR35-21.2-ss
2012-03-14    expression [ 5, 5 ] : WsR35-21.2-SeMa
2012-03-20    purification [71.0 mg]: WsR35-21.2-SeMa-Ni
2012-03-21    purification [98.4 mg]: WsR35-21.2-SeMa-GF
2012-03-28     aggregation screening [dimer,81.04 kD,97.5%]
    WsR35.001 @ HWI; 0.45 ml at 10.36 mg/mL
    WsR35.002 @ Columbia; 2 ml at 10.36 mg/mL
2013-01-04     X-Ray structure: 4ina (PDB)
    WsR35.003 @ Rutgers; 5.3 ml at 10.36 mg/mL
2013-05-21     WsR35.008 @ Columbia; 0.75 ml at 10.36 mg/mL
2013-05-21     WsR35.007 @ HWI; 0.45 ml at 10.36 mg/mL
2011-08-16    construct: WsR35-21.1
2011-09-19    expression [ 5, 5 ] : WsR35-21.1-ss
2012-08-08    construct: WsR35-TEV.3
2012-08-28    expression [ 5, 5 ] : WsR35-TEV.3-ss
2012-09-06    expression [ 5, 3 ] : WsR35-TEV.3-SeM-R1
2012-08-08    construct: WsR35-15.6
2012-08-28    expression [ 5, 5 ] : WsR35-15.6-ss
2012-09-06    expression [ 5, 5 ] : WsR35-15.6-SeM-R1
2012-09-18    purification [45.6 mg]: WsR35-15.6-SeM-R1-Ni
2012-09-19    purification [59.4 mg]: WsR35-15.6-SeM-R1-GF
2012-09-25     aggregation screening [dimer,90.39 kD,98.6%]
2012-09-26     WsR35.005 @ Columbia; 2 ml at 10.54 mg/mL
2012-09-26     WsR35.004 @ HWI; 0.45 ml at 10.54 mg/mL
2012-09-26     WsR35.006 @ Rutgers; 0.8 ml at 10.54 mg/mL
2013-05-21     WsR35.009 @ HWI; 0.45 ml at 10.54 mg/mL
2013-05-21     WsR35.010 @ Columbia; 0.75 ml at 10.54 mg/mL
2012-08-08    construct: WsR35-15.5
2012-08-28    expression [ 5, 5 ] : WsR35-15.5-ss
2012-10-10    expression [ 5, 5 ] : WsR35-15.5-SeMa
2012-08-08    construct: WsR35-TEV.4
2012-08-28    expression [ 5, 5 ] : WsR35-TEV.4-ss


Available samples:

SeMet samples
WsR35.005 @ Columbia WsR35.001 @ HWI WsR35.007 @ HWI WsR35.009 @ HWI WsR35.008 @ Columbia
WsR35.006 @ Rutgers WsR35.002 @ Columbia WsR35.010 @ Columbia WsR35.004 @ HWI WsR35.003 @ Rutgers


E-mail communications associated with this target:

  2012-03-28
  2012-04-04
  2012-09-26
  2012-09-27
  2012-10-03
  2013-05-22
  2013-05-22
  2013-05-23
  2013-05-23
  2013-05-29
  2013-05-29