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YR313A   (Nfu1)

4 targets assoicated with this target.
YR313Bsibling(60-256)selected
YR313Dsibling(147-230)soluble construct with excellent ES score
YR313Csibling(140-233)purified
YR313parentaggregation screening

database refs UniProt: P32860 (NFU1_YEAST)   |   NCBI gene: 853826   |   refSeq: NP_012884.1
status NMR structure
structures 2LTL (NMR)  
properties length: 99 aa   |   mass: 11.21 kD   |   pI: 5.52   |   ext: 6990   |   organism: Saccharomyces cerevisiae
classes PSI-Biology
projects NFU1     MPP biomedical targets     PSI:Biology Partner - MPP     MPP nominated projects    
best PDB match calculating ...
informatics Dismeta report   |   Zn binding sites: no sites identified. CH type (data) CHDE type (data)
domain signatures3.30.1370.70 details   110836 details   PF08712 details  
subsequence details   ie 100-200    
BLAST searches

Construct coverage schematic
Display constructs only from this target (YR313A).
Display constructs associated with this target (YR313A) and all other associated targets (YR313, YR313D, YR313C, YR313B).


Domain and UniProt Features
    CATH 3.30.1370.70
    SuperFamily 117916
    PFAM PF01106
    CxxC motif domain_number-1


NESG targets
    YR313A
    YR313B
    YR313C
    YR313D

YR313

       PDB seq homology



Constructs and Most Advanced Status
   
YR313-1-235-Av6HT.*

   
YR313-15.*

   
YR313A-1-130-15.*

   
YR313A-1-119-15.*

   
YR313-1-235-15.*

   
YR313A-1-124-15.*

   
YR313A-17-119-15.*

   
YR313A-17-124-15.*

   
YR313A-24-124-15.*

   
YR313A-24-130-15.*

   
YR313A-29-119-15.*

   
YR313A-29-124-15.*

   
YR313C-142-235-15.*

   
YR313C-142-235-Av6HT.*

   
YR313D-149-230-15.*

   
YR313D-149-235-Av6HT.*

   
YR313D-149-235-15.*

   
YR313D-149-230-Av6HT.*



most recent record
3 years, 25 days ago --     HSQC [not scored]

2010-11-19    target: YR313A
2011-01-10    construct: YR313A-1-130-15.1
2011-02-04    expression [ 5, 1 ] : YR313A-1-130-15.1-ss
2011-01-10    construct: YR313A-1-130-15.2
2011-02-04    expression [ 5, 1 ] : YR313A-1-130-15.2-ss
2011-01-10    construct: YR313A-1-124-15.3
2011-02-04    expression [ 5, 2 ] : YR313A-1-124-15.3-ss
2012-10-31    expression [ 5, 1 ] : YR313A-1-124-15.3-SeMa
2012-10-31    expression [ 5, 1 ] : YR313A-1-124-15.3-SeMc
2012-10-31    expression [ 5, 1 ] : YR313A-1-124-15.3-NC5c
2012-10-31    expression [ 5, 1 ] : YR313A-1-124-15.3-NC5b
2012-10-31    expression [ 5, 1 ] : YR313A-1-124-15.3-SeMb
2012-10-31    expression [ 5, 1 ] : YR313A-1-124-15.3-NC5a
2012-12-18    purification [0.5 mg]: YR313A-1-124-15.3-[NC5a,NC5b,NC5c]-GF
2013-01-02     YR313A.046 @ Rutgers; 0.096 ml at 0.36 mM
2013-01-02     YR313A.045 @ Rutgers; 0.04 ml at 0.36 mM
2014-04-28    HSQC [not scored]: YR313A.045.600MHz.20C
2012-12-17    purification [9.1 mg]: YR313A-1-124-15.3-[NC5a,NC5b,NC5c]-Ni
2011-01-10    construct: YR313A-1-124-15.4
2011-02-04    expression [ 5, 2 ] : YR313A-1-124-15.4-ss
2011-01-10    construct: YR313A-1-119-15.5
2011-02-04    expression [ 4, 2 ] : YR313A-1-119-15.5-ss
2011-01-10    construct: YR313A-1-119-15.6
2011-02-04    expression [ 5, 2 ] : YR313A-1-119-15.6-ss
2012-10-31    expression [ 5, 2 ] : YR313A-1-119-15.6-SeMb
2012-10-31    expression [ 5, 3 ] : YR313A-1-119-15.6-SeMa
2012-10-31    expression [ 5, 3 ] : YR313A-1-119-15.6-NC5b
2012-10-31    expression [ 5, 3 ] : YR313A-1-119-15.6-NC5c
2012-10-31    expression [ 5, 3 ] : YR313A-1-119-15.6-NC5a
2012-12-03    purification [2.2 mg]: YR313A-1-119-15.6-[NC5a,NC5b,NC5c]-GF
2012-12-03    purification [8.0 mg]: YR313A-1-119-15.6-[NC5a,NC5b,NC5c]-Ni
2012-10-31    expression [ 5, 2 ] : YR313A-1-119-15.6-SeMc
2011-01-10    construct: YR313A-17-124-15.7
2011-02-04    expression [ 5, 5 ] : YR313A-17-124-15.7-ss
2011-03-01    expression [ 5, 3 ] : YR313A-17-124-15.7-NC5a
2011-03-14    purification [36.4 mg]: YR313A-17-124-15.7-NC5a-Ni
2011-03-15    purification [0.7 mg]: YR313A-17-124-15.7-NC5a-GF
2011-03-23     YR313A.011 @ Rutgers; 0.04 ml at 0.572 mM
2011-05-10    HSQC [good]: YR313A.011.600MHz.20C
2011-03-23     YR313A.012 @ Rutgers; 0.52 ml at 0.572 mM
2011-07-08     YR313A.024 @ Rutgers; 0.04 ml at 0.572 mM
2011-07-08     YR313A.019 @ Rutgers; 0.04 ml at 0.572 mM
2011-07-12     YR313A.018 @ Rutgers; 0.04 ml at 0.57 mM
2011-08-04    HSQC [poor]: YR313A.018.600MHz.20C
2011-07-12     YR313A.020 @ Rutgers; 0.04 ml at 0.24 mM
2011-08-04    HSQC [good]: YR313A.020.600MHz.20C
2011-07-12     YR313A.013 @ Rutgers; 0.04 ml at 0.27 mM
2011-08-04    HSQC [good]: YR313A.013.600MHz.20C
2011-07-08     YR313A.014 @ Rutgers; 0.04 ml at 0.572 mM
2011-07-08     YR313A.015 @ Rutgers; 0.04 ml at 0.572 mM
2011-07-12     YR313A.016 @ Rutgers; 0.04 ml at 0.44 mM
2011-08-04    HSQC [poor]: YR313A.016.600MHz.20C
2011-07-12     YR313A.023 @ Rutgers; 0.04 ml at 0.25 mM
2011-08-04    HSQC [poor]: YR313A.023.600MHz.20C
2011-07-08     YR313A.021 @ Rutgers; 0.04 ml at 0.572 mM
2011-07-08     YR313A.022 @ Rutgers; 0.04 ml at 0.572 mM
2011-07-08     YR313A.017 @ Rutgers; 0.04 ml at 0.572 mM
2011-08-17    expression [ 5, 4 ] : YR313A-17-124-15.7-NCa
2011-08-24    purification [100.5 mg]: YR313A-17-124-15.7-NCa-Ni
2011-08-25    purification [7.2 mg]: YR313A-17-124-15.7-NCa-GF
2011-09-01     YR313A.037 @ Rutgers; 0.3 ml at 1.027 mM
2011-09-01     YR313A.038 @ Rutgers; 1 ml at 1.027 mM
2011-09-15    HSQC [good]: YR313A.038.600MHz.25C
2012-05-29     NMR structure: 2ltl (PDB)
2011-08-24    expression [ 5, 4 ] : YR313A-17-124-15.7-NC5b
2011-09-01    purification [0.0 mg]: YR313A-17-124-15.7-NC5b-Ni
2011-09-02    purification [7.8 mg]: YR313A-17-124-15.7-NC5b-GF
2011-09-19     YR313A.039 @ UGA; 0.5 ml at 0.891 mM
2012-01-19    expression [ 5, 4 ] : YR313A-17-124-15.7-NC5c
2012-02-02    purification [30.0 mg]: YR313A-17-124-15.7-NC5c-Ni
2012-02-02    purification [14.0 mg]: YR313A-17-124-15.7-NC5c-GF
2012-02-06     YR313A.040 @ Rutgers; 0.5 ml at 0.52 mM
2012-02-06     YR313A.041 @ Rutgers; 0.425 ml at 0.52 mM
2012-04-10     yr313a.044 @ MPP; 0.075 ml at 0.52 mM
2012-01-31    expression [ 5, 4 ] : YR313A-17-124-15.7-NC5e
2012-03-12    purification [81.8 mg]: YR313A-17-124-15.7-NC5e-Ni
2012-03-12    purification [27.6 mg]: YR313A-17-124-15.7-NC5e-GF
2012-01-31    expression [ 5, 4 ] : YR313A-17-124-15.7-NC5f
2012-03-12    purification [81.8 mg]: YR313A-17-124-15.7-NC5f-Ni
2012-03-12    purification [27.6 mg]: YR313A-17-124-15.7-NC5f-GF
2012-01-31    expression [ 5, 4 ] : YR313A-17-124-15.7-NC5d
2012-03-12    purification [81.8 mg]: YR313A-17-124-15.7-NC5d-Ni
2012-03-12    purification [27.6 mg]: YR313A-17-124-15.7-NC5d-GF
2012-04-10     YR313A.042 @ Rutgers; 1 ml at 0.68 mM
2012-04-10     YR313A.043 @ Rutgers; 0.5 ml at 0.68 mM
2011-01-10    construct: YR313A-24-130-15.9
2011-02-04    expression [ 4, 5 ] : YR313A-24-130-15.9-ss
2011-02-11    expression [ 3, 5 ] : YR313A-24-130-15.9-NC5a
2011-02-22    purification [63.3 mg]: YR313A-24-130-15.9-NC5a-Ni
2011-02-24    purification [26.1 mg]: YR313A-24-130-15.9-NC5a-GF
2011-03-03     YR313A.009 @ Rutgers; 0.04 ml at 0 mM
2011-03-09    HSQC [promising]: YR313A.009.600MHz.20C
2011-03-03     YR313A.010 @ Rutgers; 0.27 ml at 0 mM
2011-07-12     YR313A.033 @ Rutgers; 0.04 ml at 0.54 mM
2011-08-04    HSQC [good]: YR313A.033.600MHz.20C
2011-07-12     YR313A.035 @ Rutgers; 0.04 ml at 1.07 mM
2011-08-04    HSQC [poor]: YR313A.035.600MHz.20C
2011-07-12     YR313A.029 @ Rutgers; 0.04 ml at 0.86 mM
2011-08-04    HSQC [good]: YR313A.029.600MHz.20C
2011-07-08     YR313A.034 @ Rutgers; 0.04 ml at 0 mM
2011-07-12     YR313A.027 @ Rutgers; 0.04 ml at 2.69 mM
2011-08-04    HSQC [good]: YR313A.027.600MHz.20C
2011-07-12     YR313A.026 @ Rutgers; 0.04 ml at 1 mM
2011-08-04    HSQC [good]: YR313A.026.600MHz.20C
2011-07-12     YR313A.025 @ Rutgers; 0.04 ml at 0.6 mM
2011-08-04    HSQC [good]: YR313A.025.600MHz.20C
2011-07-12     YR313A.028 @ Rutgers; 0.04 ml at 1.49 mM
2011-08-04    HSQC [promising]: YR313A.028.600MHz.20C
2011-07-12     YR313A.030 @ Rutgers; 0.04 ml at 1.57 mM
2011-08-04    HSQC [promising]: YR313A.030.600MHz.20C
2011-07-12     YR313A.032 @ Rutgers; 0.04 ml at 2.82 mM
2011-08-04    HSQC [good]: YR313A.032.600MHz.20C
2011-07-08     YR313A.031 @ Rutgers; 0.04 ml at 0 mM
2011-07-08     YR313A.036 @ Rutgers; 0.04 ml at 0 mM
2011-01-10    construct: YR313A-24-130-15.10
2011-02-04    expression [ 0, 0 ] : YR313A-24-130-15.10-ss
2011-01-10    construct: YR313A-17-119-15.12
2011-02-04    expression [ 5, 4 ] : YR313A-17-119-15.12-ss
2011-02-11    expression [ 4, 4 ] : YR313A-17-119-15.12-NC5a
2011-02-22    purification [390.1 mg]: YR313A-17-119-15.12-NC5a-Ni
2011-02-24    purification [57.5 mg]: YR313A-17-119-15.12-NC5a-GF
2011-03-03     YR313A.003 @ Rutgers; 0.04 ml at 0 mM
2011-03-09    HSQC [poor]: YR313A.003.600MHz.20C
2011-03-03     YR313A.004 @ Rutgers; 0.6 ml at 0 mM
2011-01-10    construct: YR313A-24-124-15.13
2011-02-04    expression [ 4, 5 ] : YR313A-24-124-15.13-ss
2011-02-11    expression [ 4, 5 ] : YR313A-24-124-15.13-NC5a
2011-02-22    purification [120.6 mg]: YR313A-24-124-15.13-NC5a-Ni
2011-02-24    purification [68.7 mg]: YR313A-24-124-15.13-NC5a-GF
2011-03-03     YR313A.007 @ Rutgers; 0.04 ml at 0 mM
2011-03-09    HSQC [poor]: YR313A.007.600MHz.20C
2011-03-03     YR313A.008 @ Rutgers; 4.5 ml at 0 mM
2011-01-10    construct: YR313A-24-124-15.14
2011-02-04    expression [ 4, 5 ] : YR313A-24-124-15.14-ss
2011-01-10    construct: YR313A-29-124-15.15
2011-02-04    expression [ 4, 4 ] : YR313A-29-124-15.15-ss
2011-02-11    expression [ 3, 5 ] : YR313A-29-124-15.15-NC5a
2011-02-22    purification [149.9 mg]: YR313A-29-124-15.15-NC5a-Ni
2011-02-24    purification [61.8 mg]: YR313A-29-124-15.15-NC5a-GF
2011-03-03     YR313A.001 @ Rutgers; 0.04 ml at 0 mM
2011-03-09    HSQC [poor]: YR313A.001.600MHz.20C
2011-03-03     YR313A.002 @ Rutgers; 4 ml at 0 mM
2011-02-11    expression [ 3, 5 ] : YR313A-29-124-15.15-NC5b
2011-02-22    purification [149.9 mg]: YR313A-29-124-15.15-NC5b-Ni
2011-02-24    purification [61.8 mg]: YR313A-29-124-15.15-NC5b-GF
2012-06-29    expression [ 4, 5 ] : YR313A-29-124-15.15-SeMa
2012-09-25    purification [100.0 mg]: YR313A-29-124-15.15-[SeMa,SeMb]-Ni
2012-09-26    purification [0.0 mg]: YR313A-29-124-15.15-[SeMa,SeMb]-GF
2012-06-29    expression [ 4, 5 ] : YR313A-29-124-15.15-SeMb
2011-01-10    construct: YR313A-29-124-15.16
2011-02-04    expression [ 4, 5 ] : YR313A-29-124-15.16-ss
2011-01-10    construct: YR313A-29-119-15.17
2011-02-04    expression [ 4, 5 ] : YR313A-29-119-15.17-ss
2011-01-10    construct: YR313A-29-119-15.18
2011-02-04    expression [ 4, 5 ] : YR313A-29-119-15.18-ss
2011-02-11    expression [ 3, 5 ] : YR313A-29-119-15.18-NC5a
2011-02-22    purification [62.8 mg]: YR313A-29-119-15.18-NC5a-Ni
2011-02-24    purification [25.6 mg]: YR313A-29-119-15.18-NC5a-GF
2011-03-03     YR313A.005 @ Rutgers; 0.04 ml at 0 mM
2011-03-09    HSQC [poor]: YR313A.005.600MHz.20C
2011-03-03     YR313A.006 @ Rutgers; 1.25 ml at 0 mM
2012-06-29    expression [ 4, 5 ] : YR313A-29-119-15.18-SeMa
2011-01-11    construct: YR313A-17-124-15.20
2011-02-04    expression [ 5, 5 ] : YR313A-17-124-15.20-ss
2011-01-11    construct: YR313A-17-119-15.23
2011-02-04    expression [ 0, 0 ] : YR313A-17-119-15.23-ss


Available samples:

NC samples
YR313A.038 @ Rutgers YR313A.037 @ Rutgers


NC5 samples
YR313A.019 @ Rutgers yr313a.044 @ MPP YR313A.039 @ UGA
YR313A.021 @ Rutgers YR313A.012 @ Rutgers YR313A.007 @ Rutgers YR313A.020 @ Rutgers YR313A.033 @ Rutgers
YR313A.024 @ Rutgers YR313A.016 @ Rutgers YR313A.014 @ Rutgers YR313A.006 @ Rutgers YR313A.009 @ Rutgers
YR313A.013 @ Rutgers YR313A.030 @ Rutgers YR313A.018 @ Rutgers YR313A.031 @ Rutgers YR313A.032 @ Rutgers
YR313A.045 @ Rutgers YR313A.043 @ Rutgers YR313A.023 @ Rutgers YR313A.003 @ Rutgers YR313A.002 @ Rutgers
YR313A.035 @ Rutgers YR313A.027 @ Rutgers YR313A.005 @ Rutgers YR313A.029 @ Rutgers YR313A.010 @ Rutgers
YR313A.017 @ Rutgers YR313A.001 @ Rutgers YR313A.046 @ Rutgers YR313A.008 @ Rutgers YR313A.042 @ Rutgers
YR313A.015 @ Rutgers YR313A.040 @ Rutgers YR313A.004 @ Rutgers YR313A.041 @ Rutgers YR313A.025 @ Rutgers
YR313A.034 @ Rutgers YR313A.026 @ Rutgers YR313A.011 @ Rutgers YR313A.028 @ Rutgers YR313A.022 @ Rutgers
YR313A.036 @ Rutgers


E-mail communications associated with this target:

  2012-04-18