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YT682A   (SDH5)

1 targets assoicated with this target.
YT682parentgood HSQC collected

database refs UniProt: Q08230 (SDHF2_YEAST)   |   NCBI gene: 854083   |   refSeq: NP_014570.1
status NMR structure
structures 2LM4 (NMR)  
properties length: 86 aa   |   mass: 10.34 kD   |   pI: 6.51   |   ext: 23950   |   organism: Saccharomyces cerevisiae
classes PSI-1, PSI-2, MEGA, MPP: Biomedical, MPP: Community Outreach, PSI-Biology
projects SDH5     PSI:Biology Partner - MPP     MPP nominated projects     MPP biomedical targets    
best PDB match calculating ...
informatics Zn binding sites: no sites identified. CH type (data) CHDE type (data)
domain signatures109910 details   PF03937 details   1.10.150.250 details  
subsequence details   ie 100-200    
BLAST searches

Construct coverage schematic
Display constructs only from this target (YT682A).
Display constructs associated with this target (YT682A) and all other associated targets (YT682).


Domain and UniProt Features
    Transit - Mitochondrion domain_number-1
    UniProt TRANSIT:Mitochondrion (Potential)
    CATH 1.10.150.250
    SuperFamily 109910
    PFAM PF03937


NESG targets
    YT682A

YT682

       PDB seq homology



Constructs and Most Advanced Status
   
21935

   
YST6589_1_162

   
YT682-36-162-15.*

   
YT682-36-162-MBP3.*

   
YT682-44-162-15.*

   
YT682-44-162-MBP3.*

   
YT682A-55-152-R68A-15.*

   
YT682A-55-152-G77R-15.*

   
YT682A-55-152-15.*

   
YT682A-55-152-W113A-15.*

   
YT682-55-162-15.*

   
YT682A-55-152-Y71A-15.*

   
YT682A-55-152-Y71D-15.*

   
YT682A-55-152-R68E-15.*

   
YT682-60-162-15.*

   
YT682A-60-148-15.*



most recent record
3 years, 29 days ago --     HSQC [not scored]

2011-01-03    target: YT682A
2011-02-10    construct: YT682A-55-152-15.1
2011-02-21    expression [ 5, 5 ] : YT682A-55-152-15.1-ss
2011-04-06    expression [ 5, 5 ] : YT682A-55-152-15.1-NC5a
2011-04-20    purification [37.9 mg]: YT682A-55-152-15.1-NC5a-Ni
2011-04-23    purification [19.3 mg]: YT682A-55-152-15.1-NC5a-GF
2011-04-27     YT682A.001 @ Rutgers; 0.04 ml at 1.014 mM
2011-05-23    HSQC [good]: YT682A.001.600MHz.20C
2011-04-27     YT682A.002 @ Rutgers; 0.72 ml at 1.014 mM
2011-07-12     YT682A.010 @ Rutgers; 0.04 ml at 0.84 mM
2011-08-04    HSQC [good]: YT682A.010.600MHz.20C
2011-07-12     YT682A.005 @ Rutgers; 0.04 ml at 0.8 mM
2011-08-04    HSQC [good]: YT682A.005.600MHz.20C
2011-07-12     YT682A.016 @ Rutgers; 0.04 ml at 0.81 mM
2011-08-04    HSQC [good]: YT682A.016.600MHz.20C
2011-07-12     YT682A.013 @ Rutgers; 0.04 ml at 0.75 mM
2011-08-04    HSQC [good]: YT682A.013.600MHz.20C
2011-07-12     YT682A.014 @ Rutgers; 0.04 ml at 0.8 mM
2011-08-04    HSQC [good]: YT682A.014.600MHz.20C
2011-07-12     YT682A.008 @ Rutgers; 0.04 ml at 0.81 mM
2011-08-04    HSQC [good]: YT682A.008.600MHz.20C
2011-07-12     YT682A.007 @ Rutgers; 0.04 ml at 0.72 mM
2011-08-04    HSQC [good]: YT682A.007.600MHz.20C
2011-07-08     YT682A.015 @ Rutgers; 0.04 ml at 1.014 mM
2011-07-12     YT682A.012 @ Rutgers; 0.04 ml at 0.78 mM
2011-08-04    HSQC [good]: YT682A.012.600MHz.20C
2011-07-12     YT682A.009 @ Rutgers; 0.04 ml at 0.73 mM
2011-08-04    HSQC [promising]: YT682A.009.600MHz.20C
2011-07-12     YT682A.006 @ Rutgers; 0.04 ml at 0.8 mM
2011-08-04    HSQC [promising]: YT682A.006.600MHz.20C
2011-07-12     YT682A.011 @ Rutgers; 0.04 ml at 0.61 mM
2011-08-04    HSQC [good]: YT682A.011.600MHz.20C
2011-08-17    expression [ 5, 5 ] : YT682A-55-152-15.1-NCa
2011-08-24    purification [85.8 mg]: YT682A-55-152-15.1-NCa-Ni
2011-08-25    purification [31.9 mg]: YT682A-55-152-15.1-NCa-GF
2011-09-01     YT682A.017 @ Buffalo; 0.3 ml at 0.996 mM
2011-11-22     NMR structure: 2lm4 (PDB)
2011-09-01     YT682A.018 @ Rutgers; 1 ml at 0.996 mM
2011-09-15    HSQC [good]: YT682A.018.600MHz.25C
2012-04-25     YT682A.025 @ Buffalo; 0.5 ml at 0.996 mM
2011-08-24    expression [ 5, 5 ] : YT682A-55-152-15.1-NC5b
2011-09-01    purification [93.5 mg]: YT682A-55-152-15.1-NC5b-Ni
2011-09-02    purification [26.5 mg]: YT682A-55-152-15.1-NC5b-GF
2011-09-19     YT682A.019 @ UGA; 1 ml at 1.062 mM
2011-09-19     yt682a.020 @ Rutgers; 0.2 ml at 1.062 mM
2011-10-12     YT682A.021 @ Buffalo; 0.5 ml at 1.062 mM
2011-11-10     YT682A.022 @ Winge; 0.3 ml at 14.2 mg/mL
2012-01-10    expression [ 4, 5 ] : YT682A-55-152-15.1-N14a
2012-02-15    purification [31.2 mg]: YT682A-55-152-15.1-N14a-Ni
2012-02-15    purification [0.0 mg]: YT682A-55-152-15.1-N14a-GF
2012-02-22     aggregation screening [monomer,17.27 kD,59.8%]
2012-02-20     YT682A.023 @ Rutgers; 3 ml at 10.42 mg/mL
2012-02-20     YT682A.024 @ Rutgers; 0.8 ml at 10.42 mg/mL
2011-02-10    construct: YT682A-55-152-15.2
2011-02-21    expression [ 5, 5 ] : YT682A-55-152-15.2-ss
2011-02-10    construct: YT682A-60-148-15.3
2011-02-21    expression [ 5, 5 ] : YT682A-60-148-15.3-ss
2011-02-10    construct: YT682A-60-148-15.4
2011-02-21    expression [ 5, 5 ] : YT682A-60-148-15.4-ss
2011-04-06    expression [ 5, 5 ] : YT682A-60-148-15.4-NC5a
2011-04-25    purification [23.5 mg]: YT682A-60-148-15.4-NC5a-Ni
2011-04-25    purification [60.4 mg]: YT682A-60-148-15.4-NC5a-GF
2011-05-02     YT682A.003 @ Rutgers; 0.5 ml at 1.03 mM
2011-05-23    HSQC [good]: YT682A.003.600MHz.20C
2011-05-02     YT682A.004 @ Rutgers; 4.5 ml at 1.03 mM
2012-10-01    construct: YT682A-55-152-G77R-15.10
2012-10-25    expression [ 5, 5 ] : YT682A-55-152-G77R-15.10-NC5a
2013-01-03    purification [60.5 mg]: YT682A-55-152-G77R-15.10-NC5a-GF
2013-01-08     YT682A.038 @ Rutgers; 2.95 ml at 0.7 mM
2013-01-04     YT682A.027 @ Buffalo; 1 ml at 0.7 mM
2013-01-04     YT682A.026 @ Rutgers; 0.04 ml at 0.7 mM
2013-02-04    HSQC [good]: YT682A.026.600MHz.20C
2012-10-01    construct: YT682A-55-152-W113A-15.9
2012-10-25    expression [ 5, 5 ] : YT682A-55-152-W113A-15.9-NC5a
2013-01-03    purification [40.7 mg]: YT682A-55-152-W113A-15.9-NC5a-GF
2013-01-08     YT682A.039 @ Rutgers; 1.95 ml at 0.72 mM
2013-01-04     YT682A.029 @ Buffalo; 1 ml at 0.72 mM
2013-01-04     YT682A.028 @ Rutgers; 0.04 ml at 0.72 mM
2013-02-04    HSQC [good]: YT682A.028.600MHz.20C
2012-10-01    construct: YT682A-55-152-R68E-15.5
2012-10-25    expression [ 5, 5 ] : YT682A-55-152-R68E-15.5-NC5a
2013-01-03    purification [51.2 mg]: YT682A-55-152-R68E-15.5-NC5a-GF
2013-01-08     YT682A.040 @ Rutgers; 0.95 ml at 0.34 mM
2013-01-04     YT682A.031 @ Buffalo; 1 ml at 0.34 mM
2013-01-04     YT682A.030 @ Rutgers; 0.04 ml at 0.34 mM
2013-01-09    HSQC [promising]: YT682A.030.600MHz.20C
2012-10-01    construct: YT682A-55-152-Y71A-15.8
2012-10-25    expression [ 5, 5 ] : YT682A-55-152-Y71A-15.8-NC5a
2013-01-03    purification [52.2 mg]: YT682A-55-152-Y71A-15.8-NC5a-GF
2013-01-08     YT682A.041 @ Rutgers; 1.95 ml at 0.73 mM
2013-01-04     YT682A.033 @ Buffalo; 1 ml at 0.73 mM
2013-01-04     YT682A.032 @ Rutgers; 0.04 ml at 0.73 mM
2014-04-29    HSQC [not scored]: YT682A.032.600MHz.20C
2012-10-01    construct: YT682A-55-152-R68A-15.6
2012-10-25    expression [ 5, 4 ] : YT682A-55-152-R68A-15.6-NC5a
2013-01-03    purification [35.1 mg]: YT682A-55-152-R68A-15.6-NC5a-GF
2013-01-08     YT682A.042 @ Rutgers; 0.25 ml at 0.44 mM
2013-01-04     YT682A.035 @ Buffalo; 1 ml at 0.44 mM
2013-01-04     YT682A.034 @ Rutgers; 0.04 ml at 0.44 mM
2013-02-04    HSQC [promising]: YT682A.034.600MHz.20C
2012-10-01    construct: YT682A-55-152-Y71D-15.7
2012-10-25    expression [ 5, 3 ] : YT682A-55-152-Y71D-15.7-NC5a
2013-01-03    purification [67.1 mg]: YT682A-55-152-Y71D-15.7-NC5a-GF
2013-01-08     YT682A.043 @ Rutgers; 1.95 ml at 0.72 mM
2013-01-04     YT682A.037 @ Buffalo; 1 ml at 0.72 mM
2013-01-04     YT682A.036 @ Rutgers; 0.04 ml at 0.72 mM
2013-02-04    HSQC [good]: YT682A.036.600MHz.20C


Available samples:

NC samples
YT682A.025 @ Buffalo YT682A.017 @ Buffalo YT682A.018 @ Rutgers


NC5 samples
YT682A.019 @ UGA YT682A.027 @ Buffalo
YT682A.043 @ Rutgers YT682A.001 @ Rutgers YT682A.037 @ Buffalo YT682A.005 @ Rutgers YT682A.006 @ Rutgers
YT682A.036 @ Rutgers YT682A.012 @ Rutgers YT682A.014 @ Rutgers YT682A.009 @ Rutgers YT682A.028 @ Rutgers
YT682A.031 @ Buffalo YT682A.029 @ Buffalo YT682A.040 @ Rutgers YT682A.003 @ Rutgers YT682A.030 @ Rutgers
YT682A.039 @ Rutgers YT682A.042 @ Rutgers YT682A.033 @ Buffalo YT682A.004 @ Rutgers YT682A.011 @ Rutgers
YT682A.041 @ Rutgers YT682A.026 @ Rutgers YT682A.015 @ Rutgers YT682A.021 @ Buffalo YT682A.013 @ Rutgers
YT682A.010 @ Rutgers YT682A.034 @ Rutgers YT682A.007 @ Rutgers YT682A.035 @ Buffalo YT682A.032 @ Rutgers
yt682a.020 @ Rutgers YT682A.016 @ Rutgers YT682A.002 @ Rutgers YT682A.008 @ Rutgers YT682A.022 @ Winge
YT682A.038 @ Rutgers


N14 samples
YT682A.023 @ Rutgers YT682A.024 @ Rutgers


E-mail communications associated with this target:

  2012-04-26
  2013-01-11
  2013-01-16